Kinesin-1 sorting in axons controls the differential retraction of arbor terminals.
نویسندگان
چکیده
The ability of neurons to generate multiple arbor terminals from a single axon is crucial for establishing proper neuronal wiring. Although growth and retraction of arbor terminals are differentially regulated within the axon, the mechanisms by which neurons locally control their structure remain largely unknown. In the present study, we found that the kinesin-1 (Kif5 proteins) head domain (K5H) preferentially marks a subset of arbor terminals. Time-lapse imaging clarified that these arbor terminals were more stable than others, because of a low retraction rate. Local inhibition of kinesin-1 in the arbor terminal by chromophore-assisted light inactivation (CALI) enhanced the retraction rate. The microtubule turnover was locally regulated depending on the length from the branching point to the terminal end, but did not directly correlate with the presence of K5H. By contrast, F-actin signal values in arbor terminals correlated spatiotemporally with K5H, and inhibition of actin turnover prevented retraction. Results from the present study reveal a new system mediated by kinesin-1 sorting in axons that differentially controls stability of arbor terminals.
منابع مشابه
Kinesin-1 sorting in axons controls differential retraction of arbor terminals
The ability of neurons to generate multiple arbor terminals from a single axon is crucial for establishing proper neuronal wiring. While growth and retraction of arbor terminals are differentially regulated within the axon, the mechanisms by which neurons locally control their structure remain largely unknown. In the present study, we found that the head domain of kinesin-1 (K5H) preferentially...
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ورودعنوان ژورنال:
- Journal of cell science
دوره 129 18 شماره
صفحات -
تاریخ انتشار 2016