Kinesin-1 sorting in axons controls the differential retraction of arbor terminals.

نویسندگان

  • Takeshi Seno
  • Tatsuki Ikeno
  • Kousuke Mennya
  • Masayuki Kurishita
  • Narumi Sakae
  • Makoto Sato
  • Hiroki Takada
  • Yoshiyuki Konishi
چکیده

The ability of neurons to generate multiple arbor terminals from a single axon is crucial for establishing proper neuronal wiring. Although growth and retraction of arbor terminals are differentially regulated within the axon, the mechanisms by which neurons locally control their structure remain largely unknown. In the present study, we found that the kinesin-1 (Kif5 proteins) head domain (K5H) preferentially marks a subset of arbor terminals. Time-lapse imaging clarified that these arbor terminals were more stable than others, because of a low retraction rate. Local inhibition of kinesin-1 in the arbor terminal by chromophore-assisted light inactivation (CALI) enhanced the retraction rate. The microtubule turnover was locally regulated depending on the length from the branching point to the terminal end, but did not directly correlate with the presence of K5H. By contrast, F-actin signal values in arbor terminals correlated spatiotemporally with K5H, and inhibition of actin turnover prevented retraction. Results from the present study reveal a new system mediated by kinesin-1 sorting in axons that differentially controls stability of arbor terminals.

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Kinesin-1 sorting in axons controls differential retraction of arbor terminals

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عنوان ژورنال:
  • Journal of cell science

دوره 129 18  شماره 

صفحات  -

تاریخ انتشار 2016